Difference between revisions of "Part:BBa K3081059"
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| − | + | This part contains the coding gene of the thermo-sensitive transcription factor TcI42 under the constitutive promoter J23119. TcI42 is an upshifted variant of a well-known temperature-sensitive variant of the bacteriophage λ repressor cI (mutant cI857, here referred to as TcI) acting on a tandem pR–pL operator–promoter. The promoter induces the expression of CRISPRri system(<partinfo>BBa_K3081060</partinfo>), including dCas9 and sgRNA. The sgRNA called M+ targets a 20bp DNA sequence in <i>E.coli</i> OriC, which will arrest and inhibit the genome replication initiation. | |
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Latest revision as of 19:07, 21 October 2019
dCas9 J23119 M+ with the thermo-sensitive transcription factor TCI42
This part contains the coding gene of the thermo-sensitive transcription factor TcI42 under the constitutive promoter J23119. TcI42 is an upshifted variant of a well-known temperature-sensitive variant of the bacteriophage λ repressor cI (mutant cI857, here referred to as TcI) acting on a tandem pR–pL operator–promoter. The promoter induces the expression of CRISPRri system(BBa_K3081060), including dCas9 and sgRNA. The sgRNA called M+ targets a 20bp DNA sequence in E.coli OriC, which will arrest and inhibit the genome replication initiation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 753
Illegal NheI site found at 776
Illegal NheI site found at 5286
Illegal NheI site found at 5309 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1297
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]