Difference between revisions of "Part:BBa K3081061"

Latest revision as of 19:06, 21 October 2019

dCas9 J23119 PolyA with the thermo-sensitive transcription factor TCI42

This part contains the coding gene of the thermo-sensitive transcription factor TcI42 under the constitutive promoter J23119. TcI42 is an upshifted variant of a well-known temperature-sensitive variant of the bacteriophage λ repressor cI (mutant cI857, here referred to as TcI) acting on a tandem pR–pL operator–promoter. The promoter induces the expression of CRISPRri system(BBa_K3081007), including dCas9 and sgRNA. The sgRNA targets a 20bp DNA sequence in E.coli OriC, which will arrest and inhibit the genome replication initiation.But in this composite part, the sgRNA polyA is used as control. The right target sgRNA “M+” is described at BBa_K3081059.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 753
Illegal NheI site found at 776
Illegal NheI site found at 5286
Illegal NheI site found at 5309
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1297
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3081061 short</partinfo>
-This part contains the coding gene of the thermo-sensitive transcription factor TcI42 under the constitutive promoter J23119. TcI42 is an upshifted variant of a well-known temperature-sensitive variant of the bacteriophage λ repressor cI (mutant cI857, here referred to as TcI) acting on a tandem pR–pL operator–promoter. The promoter induces the expression of CRISPRri system(<partinfo>BBa_K3081007</partinfo>), including dCas9 and sgRNA. The sgRNA targets a 20bp DNA sequence in E. coli OriC, which will arrest and inhibit the genome replication initiation.But in this composite part, the sgRNA polyA is used as control. The right target sgRNA “M+” is described at BBa_K3081059.
+This part contains the coding gene of the thermo-sensitive transcription factor TcI42 under the constitutive promoter J23119. TcI42 is an upshifted variant of a well-known temperature-sensitive variant of the bacteriophage λ repressor cI (mutant cI857, here referred to as TcI) acting on a tandem pR–pL operator–promoter. The promoter induces the expression of CRISPRri system(<partinfo>BBa_K3081007</partinfo>), including dCas9 and sgRNA. The sgRNA targets a 20bp DNA sequence in <i>E.coli</i> OriC, which will arrest and inhibit the genome replication initiation.But in this composite part, the sgRNA polyA is used as control. The right target sgRNA “M+” is described at <partinfo>BBa_K3081059</partinfo>.
 <!-- Add more about the biology of this part here