Difference between revisions of "Part:BBa K2983005"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | The URA3 auxotrophic selection marker ([[Part:BBa_K2983005|BBa_K2983005]]) which is composed of the URA3 promoter ([[Part:BBa_K2983002|BBa_K2983002]]), URA3 gene ([[Part:BBa_K2983003|BBa_K2983003]]) and the URA3 terminator ([[Part:BBa_K2983004|BBa_K2983004]]). The | + | The URA3 auxotrophic selection marker ([[Part:BBa_K2983005|BBa_K2983005]]) which is composed of the URA3 promoter ([[Part:BBa_K2983002|BBa_K2983002]]), URA3 gene ([[Part:BBa_K2983003|BBa_K2983003]]) and the URA3 terminator ([[Part:BBa_K2983004|BBa_K2983004]]). |
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+ | The Ura3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotides synthesis pathway. In the absence of this enzyme, the cells are able to grow only if uracil or uridine are present in the media. | ||
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+ | It is a wildly used auxotrophic selection marker in yeast. | ||
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Revision as of 18:51, 21 October 2019
Ura3 auxotrophic selection marker
This part is the Ura3 auxotrophic selection marker for Yarrowia lipolytica.
Usage and Biology
The URA3 auxotrophic selection marker (BBa_K2983005) which is composed of the URA3 promoter (BBa_K2983002), URA3 gene (BBa_K2983003) and the URA3 terminator (BBa_K2983004).
The Ura3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotides synthesis pathway. In the absence of this enzyme, the cells are able to grow only if uracil or uridine are present in the media.
It is a wildly used auxotrophic selection marker in yeast.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 195
Illegal XhoI site found at 228 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 383
Illegal AgeI site found at 486 - 1000COMPATIBLE WITH RFC[1000]