Difference between revisions of "Part:BBa K2983005"

Revision as of 18:46, 21 October 2019

Ura3 auxotrophic selection marker

This part is the Ura3 auxotrophic selection marker for Yarrowia lipolytica.

Usage and Biology

The URA3 auxotrophic selection marker (BBa_K2983005) which is composed of the URA3 promoter (BBa_K2983002), URA3 gene (BBa_K2983003) and the URA3 terminator (BBa_K2983004). The URA3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotide synthesis pathway.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 195
Illegal XhoI site found at 228
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 383
Illegal AgeI site found at 486
1000
COMPATIBLE WITH RFC[1000]

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 This part is the Ura3 auxotrophic selection marker for ''Yarrowia lipolytica''.
+===Usage and Biology===
 The URA3 auxotrophic selection marker ([[Part:BBa_K2983005|BBa_K2983005]]) which is composed of the URA3 promoter ([[Part:BBa_K2983002|BBa_K2983002]]), URA3 gene ([[Part:BBa_K2983003|BBa_K2983003]]) and the URA3 terminator ([[Part:BBa_K2983004|BBa_K2983004]]). The URA3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotide synthesis pathway.