Difference between revisions of "Part:BBa K3063903"
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<partinfo>BBa_K3063903 short</partinfo> | <partinfo>BBa_K3063903 short</partinfo> | ||
| − | + | The part encodes artificial microRNA (amiRNA) that silences SOX9, a gene promoting cancer metastasis and driving cancer cell growth. <ref> Carrasco-Garcia, E., Lopez, L., Aldaz, P., Arevalo, S., Aldaregia, J., Egaña, L., ... & Matheu, A. (2016). SOX9-regulated cell plasticity in colorectal metastasis is attenuated by rapamycin. Scientific reports, 6, 32350. </ref> SOX9-targeting amiRNA in the transformed salmonella, will form dsRNA upon binding to stemness SOX9 mRNA in tumour cells. Under the aid of this particular biobrick, RNA silencing and degradation of SOX9 mRNA in liver cancer stem cells can be achieved simultaneously. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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<partinfo>BBa_K3063903 parameters</partinfo> | <partinfo>BBa_K3063903 parameters</partinfo> | ||
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| + | __TOC__ | ||
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| + | =Introduction= | ||
| + | ==Biology== | ||
| + | ===Part structure=== | ||
| + | The part for SOX9 silencing in cancer stem cells | ||
| + | |||
| + | BBa_K3063901 | ||
| + | The part for SOX9 silencing in cancer stem cells. With the help of enhanced synthetic inhibitory BIC/miR-155 RNA (eSIBR) vector, pri-miRNA hairpin as shown below is transcribed and later processed into mature miRNA in Salmonella Typhimurium that achieve SOX9 knockdown in target cancer cells. | ||
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| + | https://static.igem.org/mediawiki/parts/c/c8/T--Hong_Kong_HKU--SOX4.jpg | ||
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| + | ===Part Therapeutics=== | ||
| + | Sox9 has long been known to overexpress in tumor, in particular, cancer stem cells. It maintains self-renewal capacity of CSCs through constitutive activation of Notch signalling pathway <ref> Liu, C., Liu, L., Chen, X., Cheng, J., Zhang, H., Shen, J., ... & Qian, C. (2016). Sox9 regulates self‐renewal and tumorigenicity by promoting symmetrical cell division of cancer stem cells in hepatocellular carcinoma. Hepatology, 64(1), 117-129. </ref> Metastatic cells show further elevation of Sox9 than non-metastatic ones, indicating the role of the gene in cancer progression, possibly via Wnt/β-catenin signaling. <ref> Aguilar-Medina, M., Avendaño-Félix, M., Lizárraga-Verdugo, E., Bermúdez, M., Romero-Quintana, J. G., Ramos-Payan, R., ... & López-Camarillo, C. (2019). SOX9 Stem-Cell Factor: Clinical and Functional Relevance in Cancer. Journal of oncology, 2019. </ref> | ||
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| + | =References= | ||
Revision as of 18:45, 21 October 2019
eSIBR construct fragment for SOX9
The part encodes artificial microRNA (amiRNA) that silences SOX9, a gene promoting cancer metastasis and driving cancer cell growth. [1] SOX9-targeting amiRNA in the transformed salmonella, will form dsRNA upon binding to stemness SOX9 mRNA in tumour cells. Under the aid of this particular biobrick, RNA silencing and degradation of SOX9 mRNA in liver cancer stem cells can be achieved simultaneously.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 156
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Introduction
Biology
Part structure
The part for SOX9 silencing in cancer stem cells
BBa_K3063901 The part for SOX9 silencing in cancer stem cells. With the help of enhanced synthetic inhibitory BIC/miR-155 RNA (eSIBR) vector, pri-miRNA hairpin as shown below is transcribed and later processed into mature miRNA in Salmonella Typhimurium that achieve SOX9 knockdown in target cancer cells.
Part Therapeutics
Sox9 has long been known to overexpress in tumor, in particular, cancer stem cells. It maintains self-renewal capacity of CSCs through constitutive activation of Notch signalling pathway [2] Metastatic cells show further elevation of Sox9 than non-metastatic ones, indicating the role of the gene in cancer progression, possibly via Wnt/β-catenin signaling. [3]
References
- ↑ Carrasco-Garcia, E., Lopez, L., Aldaz, P., Arevalo, S., Aldaregia, J., Egaña, L., ... & Matheu, A. (2016). SOX9-regulated cell plasticity in colorectal metastasis is attenuated by rapamycin. Scientific reports, 6, 32350.
- ↑ Liu, C., Liu, L., Chen, X., Cheng, J., Zhang, H., Shen, J., ... & Qian, C. (2016). Sox9 regulates self‐renewal and tumorigenicity by promoting symmetrical cell division of cancer stem cells in hepatocellular carcinoma. Hepatology, 64(1), 117-129.
- ↑ Aguilar-Medina, M., Avendaño-Félix, M., Lizárraga-Verdugo, E., Bermúdez, M., Romero-Quintana, J. G., Ramos-Payan, R., ... & López-Camarillo, C. (2019). SOX9 Stem-Cell Factor: Clinical and Functional Relevance in Cancer. Journal of oncology, 2019.