Difference between revisions of "Part:BBa K2983005"
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This part is the Ura3 auxotrophic selection marker for ''Yarrowia lipolytica''.
 
This part is the Ura3 auxotrophic selection marker for ''Yarrowia lipolytica''.
  
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The URA3 auxotrophic selection marker ([[Part:BBa_K2983005|BBa_K2983005]]) which is composed of the URA3 promoter ([[BBa_K2983002|BBa_K2983002]]), URA3 gene ([[BBa_K2983003|BBa_K2983003]]) and the URA3 terminator ([[BBa_K2983004|BBa_K2983004]] ). The URA3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotide synthesis pathway.
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The URA3 auxotrophic selection marker ([[Part:BBa_K2983005|BBa_K2983005]]) which is composed of the URA3 promoter ([[BBa_K2983002|BBa_K2983002]]), URA3 gene ([[BBa_K2983003|BBa_K2983003]]) and the URA3 terminator ([[BBa_K2983004|BBa_K2983004]]). The URA3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotide synthesis pathway.
  
 
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Revision as of 18:44, 21 October 2019


Ura3 auxotrophic selection marker

This part is the Ura3 auxotrophic selection marker for Yarrowia lipolytica.

The URA3 auxotrophic selection marker (BBa_K2983005) which is composed of the URA3 promoter (BBa_K2983002), URA3 gene (BBa_K2983003) and the URA3 terminator (BBa_K2983004). The URA3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotide synthesis pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 195
    Illegal XhoI site found at 228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 486
  • 1000
    COMPATIBLE WITH RFC[1000]