Difference between revisions of "Part:BBa K3280001"
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<partinfo>BBa_K3280001 short</partinfo>
 
<partinfo>BBa_K3280001 short</partinfo>
  
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WspR. This part is a protein domain and increase levels of c-di-GMP to induce biofilm formation.
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WspR. This part is a protein that has a domain that increases levels of c-di-GMP to induce biofilm formation.
  
 
<h2>Characterization</h2>
 
<h2>Characterization</h2>
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Cyclic di-GMP (c-di-GMP) is recognized as an intracellular signaling molecule coordinating the “lifestyle transition” from motility to sessility and vice versa (i.e. dispersion). The correlation between high c-di-GMP concentration in the cell and biofilm formation or between low c-di-GMP levels and motility has been demonstrated in several bacteria species [1].
 
Cyclic di-GMP (c-di-GMP) is recognized as an intracellular signaling molecule coordinating the “lifestyle transition” from motility to sessility and vice versa (i.e. dispersion). The correlation between high c-di-GMP concentration in the cell and biofilm formation or between low c-di-GMP levels and motility has been demonstrated in several bacteria species [1].
  
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Our project include one protein (Iara-alpha) that captures mercury and also produces biofilm to improve the fixation of the heavy metal. So one of our experiments was to characterize several diguanylate cyclases in order to choose the best one for our circuit.
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Our project include one protein (Iara-alpha) that captures mercury and also produces biofilm to improve the fixation of the heavy metal. So one of our experiments was to characterize several diguanylate cyclases in order to choose the best one for our circuit.  
  
 
[[File:T--USP_SaoCarlos-Brazil--Chimera-vector.svg|700px|center]]
 
[[File:T--USP_SaoCarlos-Brazil--Chimera-vector.svg|700px|center]]
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To know the specific protocol we used, access [[https://2019.igem.org/Team:USP_SaoCarlos-Brazil/Experiments]].
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[[File:T--USP_SaoCarlos-Brazil--Parts-DGC24.png|700px|center]]
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[[File:T--USP_SaoCarlos-Brazil--Parts-DGC24.png|700px|center]]
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<h2>References</h2>
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[1]. Ha DG, O'Toole GA. c-di-GMP and its Effects on Biofilm Formation and Dispersion: a Pseudomonas Aeruginosa Review. Microbiol Spectr. 2015;3(2):10.1128/microbiolspec.MB-0003-2014. doi:10.1128/microbiolspec.MB-0003-2014
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[2] Valentini M, Filloux A. Biofilms and Cyclic di-GMP (c-di-GMP) Signaling: Lessons from Pseudomonas aeruginosa and Other Bacteria. J Biol Chem. 2016;291(24):12547–12555. doi:10.1074/jbc.R115.711507
  
  

Revision as of 18:34, 21 October 2019


WspR Diguanylate Cyclase

WspR. This part is a protein that has a domain that increases levels of c-di-GMP to induce biofilm formation.

Characterization

By Team iGEM19_USP_SaoCarlos-Brazil 2019

Cyclic di-GMP (c-di-GMP) is recognized as an intracellular signaling molecule coordinating the “lifestyle transition” from motility to sessility and vice versa (i.e. dispersion). The correlation between high c-di-GMP concentration in the cell and biofilm formation or between low c-di-GMP levels and motility has been demonstrated in several bacteria species [1].

Our project include one protein (Iara-alpha) that captures mercury and also produces biofilm to improve the fixation of the heavy metal. So one of our experiments was to characterize several diguanylate cyclases in order to choose the best one for our circuit.

Error creating thumbnail: File missing

To know the specific protocol we used, access [[1]].


T--USP SaoCarlos-Brazil--Parts-DGC24.png
T--USP SaoCarlos-Brazil--Parts-DGC24.png

References

[1]. Ha DG, O'Toole GA. c-di-GMP and its Effects on Biofilm Formation and Dispersion: a Pseudomonas Aeruginosa Review. Microbiol Spectr. 2015;3(2):10.1128/microbiolspec.MB-0003-2014. doi:10.1128/microbiolspec.MB-0003-2014

[2] Valentini M, Filloux A. Biofilms and Cyclic di-GMP (c-di-GMP) Signaling: Lessons from Pseudomonas aeruginosa and Other Bacteria. J Biol Chem. 2016;291(24):12547–12555. doi:10.1074/jbc.R115.711507


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 393
    Illegal PstI site found at 465
    Illegal PstI site found at 683
    Illegal PstI site found at 807
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 393
    Illegal PstI site found at 465
    Illegal PstI site found at 683
    Illegal PstI site found at 807
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 361
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 393
    Illegal PstI site found at 465
    Illegal PstI site found at 683
    Illegal PstI site found at 807
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 393
    Illegal PstI site found at 465
    Illegal PstI site found at 683
    Illegal PstI site found at 807
    Illegal NgoMIV site found at 306
    Illegal NgoMIV site found at 636
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 450
    Illegal SapI.rc site found at 273
    Illegal SapI.rc site found at 287