Difference between revisions of "Part:BBa K3081012"
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This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the R4- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R4 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R4 box, severe arrest and inhibition of genome replication initiation is achieved.  
 
This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the R4- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R4 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R4 box, severe arrest and inhibition of genome replication initiation is achieved.  
  
 +
<H1>Experiment</H1>
 
For more detailed information, see <partinfo>BBa_K3081058</partinfo>
 
For more detailed information, see <partinfo>BBa_K3081058</partinfo>
  

Revision as of 18:26, 21 October 2019


pBAD-dCas9-J23119-R4-

This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the R4- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R4 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R4 box, severe arrest and inhibition of genome replication initiation is achieved.

Experiment

For more detailed information, see BBa_K3081058

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961