Difference between revisions of "Part:BBa K2974500:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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<strong>Medium Promoter (BBa_J23106) Toehold GFP</strong>
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how you used this part and how it worked out.
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<br>
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In order to apply this improvement to part BBa_K2550000, Lambert iGEM re-built the T7 Toehold LacZ construct in SnapGene with part BBa_J23106 as the new promoter. The team then began the cloning workflow using Gibson Assembly, aiming to compare pigmentation of transformed E. coli containing the individual toehold and trigger, as well as a dual plasmid transformation of both. The improved part is BBa_K2974500 and is currently, as of wiki freeze, in the cloning stage, but the team expects to update the registry with the final results after the 2019 Giant Jamboree.
  
 
===Applications of BBa_K2974500===
 
===Applications of BBa_K2974500===
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This composite part can be used in measurement systems.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 18:25, 21 October 2019


Medium Promoter (BBa_J23106) Toehold GFP
In order to apply this improvement to part BBa_K2550000, Lambert iGEM re-built the T7 Toehold LacZ construct in SnapGene with part BBa_J23106 as the new promoter. The team then began the cloning workflow using Gibson Assembly, aiming to compare pigmentation of transformed E. coli containing the individual toehold and trigger, as well as a dual plasmid transformation of both. The improved part is BBa_K2974500 and is currently, as of wiki freeze, in the cloning stage, but the team expects to update the registry with the final results after the 2019 Giant Jamboree.

Applications of BBa_K2974500

This composite part can be used in measurement systems.

User Reviews

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