Difference between revisions of "Part:BBa K3046008:Design"

 
 
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===Design Notes===
 
===Design Notes===
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This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.
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===Source===
 
===Source===
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This part is created on the basis of the upstream region of the gpdA ORF (Systematic ORF name: An08g09880) of many <i>aspergillus</i> genomes. It has been created using the proHMMoter software with added noise, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model
  
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===References===
 
===References===
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[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019.

Latest revision as of 18:11, 21 October 2019


PLEAPhfbD_1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 14
    Illegal NheI site found at 676
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 436
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is optimised to be compatible with the MoClo standard, Mobius assembly standard and not to contain any SwaI cut sites.


Source

This part is created on the basis of the upstream region of the gpdA ORF (Systematic ORF name: An08g09880) of many aspergillus genomes. It has been created using the proHMMoter software with added noise, as a part of the LEAP project, described in further detail here: https://2019.igem.org/Team:DTU-Denmark/Model


References

[1] P. Schape et al., “Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks.,” Nucleic Acids Res., vol. 47, no. 2, pp. 559–569, Jan. 2019.