Difference between revisions of "Part:BBa K3183000"
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===Characterisation=== | ===Characterisation=== | ||
| − | This part was characterised in the composite parts <partinfo>BBa_K3183100</partinfo>, and <partinfo> BBa_K3183028</partinfo> . | + | This part was characterised in the composite parts <partinfo>BBa_K3183100</partinfo>, and <partinfo>BBa_K3183028</partinfo>. |
===References=== | ===References=== | ||
1. Swinfield, Tracy-Jane, et al. “Physical Characterisation of the Replication Region of the Streptococcus Faecalis Plasmid pAMβ1.” Gene, vol. 87, no. 1, 1990, pp. 79–90., doi:10.1016/s0378-1119(19)30488-3. <br> | 1. Swinfield, Tracy-Jane, et al. “Physical Characterisation of the Replication Region of the Streptococcus Faecalis Plasmid pAMβ1.” Gene, vol. 87, no. 1, 1990, pp. 79–90., doi:10.1016/s0378-1119(19)30488-3. <br> | ||
2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x. | 2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x. | ||
Revision as of 18:06, 21 October 2019
Erythromycin Constitutive Promoter
P-erm is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species. It has also been shown to be functional in E. coli.
The promoter is derived from the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAMβ1 isolated from Enterococcus faecalis.1 It was subsequently characterised in six strains of Lactobacillus reuteri and Lactococcus lactisspp. cremoris MG1363.2
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Use by Team Oxford 2019
This promoter was used in number of composite parts by Team Oxford 2019. The composite parts containing P-erm are BBa_K3183103, BBa_K3183100, and BBa_K3183028.
Characterisation
This part was characterised in the composite parts BBa_K3183100, and BBa_K3183028.
References
1. Swinfield, Tracy-Jane, et al. “Physical Characterisation of the Replication Region of the Streptococcus Faecalis Plasmid pAMβ1.” Gene, vol. 87, no. 1, 1990, pp. 79–90., doi:10.1016/s0378-1119(19)30488-3.
2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.