Difference between revisions of "Part:BBa K2940005"

Revision as of 17:59, 21 October 2019

Coding Sequence of AzoR with an N-terminal SpyCatcher

Coding sequence for Azoreductase AzoR with an N-terminal SpyCatcher.

Usage and Biology

This part is used for conjugation of AzoR on curli for enzyme immobilisation. The SpyCatcher is designed to be at the N-terminus of the AzoR enzyme. A 2x GGGGS flexible linker was introduced between AzoR and SpyCatcher to make sure the domains of two proteins were not interfering with each other. SpyCatcher sequence were acquired from iGEM registry (part: BBa_K1650037). And the flavin-free, oxygen insensitive azoreductase AzoR was acquired from NCBI (accession number: WP_014229193.1).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 222
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 Coding sequence for Azoreductase AzoR with an N-terminal SpyCatcher.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part is used for conjugation of AzoR on curli for enzyme immobilisation. The SpyCatcher is designed to be at the N-terminus of the AzoR enzyme. A 2x GGGGS flexible linker was introduced between AzoR and SpyCatcher to make sure the domains of two proteins were not interfering with each other. SpyCatcher sequence were acquired from iGEM registry (part: BBa_K1650037). And the flavin-free, oxygen insensitive azoreductase AzoR was acquired from NCBI (accession number: WP_014229193.1).
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