Difference between revisions of "Part:BBa K3081115"

Latest revision as of 17:54, 21 October 2019

a 20bp sequence targeting the R3(top strand) DnaA box on E.coli oriC

The 20 bp sgRNA targets to the R3+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R3 is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to R3 box, moderate arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058

Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]

Reference: [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3081115 short</partinfo>
-a 20bp sequence targeting the R3(top strand) DnaA box on E.coli oriC
+The 20 bp sgRNA targets to the R3+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R3 is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to R3 box, moderate arrest and inhibition of genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo>
+<center>
+https://2019.igem.org/wiki/images/0/0a/T--Peking--box.png
+</center>
+<center>
+Figure1: Different boxes related to <i>E.coli</i> genome replication.This figure is adapted from the review of Costa, A. et al[1]
+</center>
+Reference:
+[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
 <!-- Add more about the biology of this part here