Difference between revisions of "Part:BBa K3285700"
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pbrD is a protein that sequesters lead. It is present downstream of a strong RBS (BBa_B0034) and binds to lead ions in the cytoplasm and decrease its concentration. | pbrD is a protein that sequesters lead. It is present downstream of a strong RBS (BBa_B0034) and binds to lead ions in the cytoplasm and decrease its concentration. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This is an intermediate part which can be incorporated under a promoter. The pbrD protein is known to sequester lead ions in the cytoplasm of the bacterial cell. | ||
| + | |||
| + | When put under a lead responsive promoter, the part is expected to show dosage dependent sequestration properties. | ||
| + | |||
| + | ===Construction=== | ||
| + | |||
| + | "https://2019.igem.org/wiki/images/d/d2/T--IISER-Pune-India--pbrD_Gel.jpeg" | ||
| + | <p> | ||
| + | The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor [https://parts.igem.org/Part:BBa_K1758333 BBa_K1758333]. This part has been synthesised with a strong Ribosome Binding Site [https://parts.igem.org/Part:BBa_B0034 BBa_B0034] upstream of the coding sequence. | ||
| + | </p> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 17:49, 21 October 2019
Lead Sequestration Protein with RBS
pbrD is a protein that sequesters lead. It is present downstream of a strong RBS (BBa_B0034) and binds to lead ions in the cytoplasm and decrease its concentration.
Usage and Biology
This is an intermediate part which can be incorporated under a promoter. The pbrD protein is known to sequester lead ions in the cytoplasm of the bacterial cell.
When put under a lead responsive promoter, the part is expected to show dosage dependent sequestration properties.
Construction
"
"
The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor BBa_K1758333. This part has been synthesised with a strong Ribosome Binding Site BBa_B0034 upstream of the coding sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 532
- 1000COMPATIBLE WITH RFC[1000]