Difference between revisions of "Part:BBa K2984053"
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>Our carbonic anhydrase constructs are supposed to enhance chlamydomanas reinhardtii´s capability to use carbonic acid as a carbon source. The Aminoacid-sequence of our carbonic anhydrase was obtained via uniprot. The enzyme catalyzes the reaction depicted below. Chararacterization of this part may be possible by using media with different concentrations of carbonic acid in a algae growth measurement. Sadly we didn´t have time to plan and carry out any transformations or characterizations with this part.
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This sequence also contains two rbcS2i1 introns inserted as suggest by Baier et al, 2018. </span>
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<img src="https://2019.igem.org/wiki/images/5/5d/T--Humboldt_Berlin--CarbonicAnhydrase.png" width="600"/>
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<partinfo>BBa_K2984053 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984053 SequenceAndFeatures</partinfo>
  

Latest revision as of 17:44, 21 October 2019


Carbonicanhydrase

Carbonicanhydrase B3-B4

Our carbonic anhydrase constructs are supposed to enhance chlamydomanas reinhardtii´s capability to use carbonic acid as a carbon source. The Aminoacid-sequence of our carbonic anhydrase was obtained via uniprot. The enzyme catalyzes the reaction depicted below. Chararacterization of this part may be possible by using media with different concentrations of carbonic acid in a algae growth measurement. Sadly we didn´t have time to plan and carry out any transformations or characterizations with this part. This sequence also contains two rbcS2i1 introns inserted as suggest by Baier et al, 2018.




Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 280
    Illegal PstI site found at 627
    Illegal PstI site found at 1000
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 280
    Illegal PstI site found at 627
    Illegal PstI site found at 1000
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 259
    Illegal XhoI site found at 445
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 280
    Illegal PstI site found at 627
    Illegal PstI site found at 1000
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 280
    Illegal PstI site found at 627
    Illegal PstI site found at 1000
    Illegal NgoMIV site found at 382
    Illegal NgoMIV site found at 1269
    Illegal AgeI site found at 1210
  • 1000
    COMPATIBLE WITH RFC[1000]