Difference between revisions of "Part:BBa K3320003"
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part is repressed by BBa_K3320019 and K3320016 in absence of Lactate and Hypoxia, physiological characteristics from the tumor microenvironment. When induced, it expresses the BBa_K3320013 to be secreted directly from the cytosol into the extracellular environment by the alpha-hemolysin secretion system. | This part is repressed by BBa_K3320019 and K3320016 in absence of Lactate and Hypoxia, physiological characteristics from the tumor microenvironment. When induced, it expresses the BBa_K3320013 to be secreted directly from the cytosol into the extracellular environment by the alpha-hemolysin secretion system. | ||
− | + | ===Assembly=== | |
+ | <p>This part were cloned on the pUC19 vector, totaling 3752 bp. </p> | ||
+ | [[File:BBa_K3320003_Map.png|500px|]] | ||
+ | Figure 1. BBa_K3320003 plasmidial map.png | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3320003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3320003 SequenceAndFeatures</partinfo> |
Revision as of 17:41, 21 October 2019
pTet + Ellowitz RBS + GFPMut3 + L3S2P22
This composite part produce GFPMut3 induced by pTet promoter.
Usage and Biology
This part is repressed by BBa_K3320019 and K3320016 in absence of Lactate and Hypoxia, physiological characteristics from the tumor microenvironment. When induced, it expresses the BBa_K3320013 to be secreted directly from the cytosol into the extracellular environment by the alpha-hemolysin secretion system.
Assembly
This part were cloned on the pUC19 vector, totaling 3752 bp.
Figure 1. BBa_K3320003 plasmidial map.png Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]