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In order to determine the strength of this promoter at different temperature and glucose concentration, we constructed a plasmid with the expression of GFP as an indicator downstream the TEF1 promoter and measured its intensity of fluorescence. Additionally， we also constructed a plasmid with GFP only as a negative control.  
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In order to determine the strength of this promoter at different temperature and glucose concentration, we constructed a plasmid with the expression of GFP as an indicator downstream the TEF1 promoter and measured its intensity of fluorescence. Additionally, we also constructed a plasmid with GFP only as a negative control.  
  
 
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Latest revision as of 17:41, 21 October 2019


TEF1 promoter

A promoter

Contribution: BNDS_China 2019

Group: BNDS_China iGEM 2019
Author: Wanji Li
Introduction & Design:

In order to determine the strength of this promoter at different temperature and glucose concentration, we constructed a plasmid with the expression of GFP as an indicator downstream the TEF1 promoter and measured its intensity of fluorescence. Additionally, we also constructed a plasmid with GFP only as a negative control.



FIGURE 1(A) the intensity of fluorescence under different concentration of glucose in 30 degree Celsius; (B) the intensity of fluorescence under different concentration of glucose in 32 degree Celsius; (C) the intensity of fluorescence under different concentration of glucose in 34 degree Celsius; (D) the intensity of fluorescence under different concentration of glucose in 37 degree Celsius


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]