Difference between revisions of "Part:BBa K592002"
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− | + | [[File:T--NYU_New_York--Ccar1.png|600px|thumb|left|Ccar1]] | |
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<p>Figure 1: Plates containing the pSR58.6 (left) and pNO286-3 (right) plasmids under UV light. The pSR58.6 plasmid contains CcaR and superfolder GFP. The pNO286-3 plasmid contains the mini-CcaS gene, along with ho1 and pcyA.</p> | <p>Figure 1: Plates containing the pSR58.6 (left) and pNO286-3 (right) plasmids under UV light. The pSR58.6 plasmid contains CcaR and superfolder GFP. The pNO286-3 plasmid contains the mini-CcaS gene, along with ho1 and pcyA.</p> | ||
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Revision as of 17:33, 21 October 2019
ccaR
ccaR is the response regulator of the green sensor (BBa_K592001) that also includes the membrane-associated histidine kinase ccaS. The two-component system is also dependent of a chromophore.
Figure 1: Plates containing the pSR58.6 (left) and pNO286-3 (right) plasmids under UV light. The pSR58.6 plasmid contains CcaR and superfolder GFP. The pNO286-3 plasmid contains the mini-CcaS gene, along with ho1 and pcyA.
<img src=“https://2019.igem.org/wiki/images/a/a3/T--NYU_New_York--results2.jpg”>
Figure 2: Plate containing cells co-transformed with both pSR58.6 and pNO286-3 plasmids.
<img src=“https://2019.igem.org/wiki/images/6/60/T--NYU_New_York--results3.png”>
Figure 3: Light sensor readings from plates containing optogenetic plasmids
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 402
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]