Difference between revisions of "Part:BBa K3183025"
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===References===
 
===References===
  
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Tobias, J., et al. “The N-End Rule in Bacteria.” Science, vol. 254, no. 5036, 1991, pp. 1374–1377., doi:10.1126/science.1962196.
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1. Tobias, J., et al. “The N-End Rule in Bacteria.” Science, vol. 254, no. 5036, 1991, pp. 1374–1377., doi:10.1126/science.1962196.

Latest revision as of 17:25, 21 October 2019


Start Methionine-Glycine-Serine Linker Codon-Optimized for E. coli

Short hydrophilic linker containing the initiator codon, immediately followed by two residues. These residues have been shown to enhance the lifetime of the protein in the bacterial cytoplasm when placed at the N-terminus, after Met.1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Tobias, J., et al. “The N-End Rule in Bacteria.” Science, vol. 254, no. 5036, 1991, pp. 1374–1377., doi:10.1126/science.1962196.