Difference between revisions of "Part:BBa K3228054"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3228054 short</partinfo>
 
<partinfo>BBa_K3228054 short</partinfo>
  
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test
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===Sequence and Features===
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<partinfo>BBa_K3228054 SequenceAndFeatures</partinfo>
  
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<!-- Add more about the biology of this part here
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===Description===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3228054 SequenceAndFeatures</partinfo>
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This part contains the ribosomal binding site <a href="https://parts.igem.org/Part:BBa_B0034"> B0034</a> and riboJ which is a ribozyme. RiboJ comprises the ribozyme with an additional hairpin to help expose the RBS. RiboJ contains both the ribozyme and hairpin <a href="https://www.nature.com/articles/nbt.2401">(Lou, Stanton, Chen, Munsky, & Voigt, 2012)</a>.
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Upon translation of the ribozyme into RNA, it cleaves itself off, removing any transcript from the mRNA upstream of the ribosomal binding site (RBS).
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<div style="width:100%;display:flex;flex-direction:row;flex-wrap: wrap; justify-content:space-evenly; align-items:center;">[[File:GE riboinsulators.png|500px|thumb|left|'''Figure 1''': Maturation of mRNA transcripts leads to a more standardized genetic context.  ]]
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This leads to a more stable expression independent of the cellular context <a href="http://2016.igem.org/Team:William_and_Mary/RiboJ">(See iGEM William and Mary 2016)</a> and can be used for better cross comparison of results over species border or the construction of more robust, species independent functional devices.
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===Compability===
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These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design"> Design of the Marburg Collection</a>.
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===Available Ribo-insulators===
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<div class="PCListIcon" style="display:flex;flex-direction:row; flex-wrap: nowrap; justify-content:space-evenly; align-items:flex-start;font-size:82%;">
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[[File:Icon Ribo Isulator.png|90px|thumb|none|
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<li> <a href="https://parts.igem.org/Part:BBa_K3228054">K3228054 </a> (riboJ B0034) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228055">K3228055 </a> (riboJ B0034 <br> no Scar) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228056">K3228056 </a> <br> (SarJ B0034) </li>
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<li> <a href="https://parts.igem.org/Part:BBa_K3228057">K3228057 </a> (PlmJ B0034) </li>
  
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===Functional Parameters===
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<partinfo>BBa_K3228054 parameters</partinfo>
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Latest revision as of 17:23, 21 October 2019


pMC_0_3_12_riboJ_B0034

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

This part contains the ribosomal binding site B0034 and riboJ which is a ribozyme. RiboJ comprises the ribozyme with an additional hairpin to help expose the RBS. RiboJ contains both the ribozyme and hairpin (Lou, Stanton, Chen, Munsky, & Voigt, 2012).

Upon translation of the ribozyme into RNA, it cleaves itself off, removing any transcript from the mRNA upstream of the ribosomal binding site (RBS).

Figure 1: Maturation of mRNA transcripts leads to a more standardized genetic context.

This leads to a more stable expression independent of the cellular context (See iGEM William and Mary 2016) and can be used for better cross comparison of results over species border or the construction of more robust, species independent functional devices.

Compability

These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.

Available Ribo-insulators