Difference between revisions of "Part:BBa K3320003"
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This composite part produce GFPMut3 induced by pTet promoter. | This composite part produce GFPMut3 induced by pTet promoter. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here <!-- --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part is repressed by BBa_K3320019 and K3320016 in absence of Lactate and Hypoxia, physiological characteristics from the tumor microenvironment. When induced, it expresses the BBa_K3320013 to be secreted directly from the cytosol into the extracellular environment by the alpha-hemolysin secretion system. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3320003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3320003 SequenceAndFeatures</partinfo> | ||
Revision as of 17:23, 21 October 2019
pTet + Ellowitz RBS + GFPMut3 + L3S2P22
This composite part produce GFPMut3 induced by pTet promoter.
Usage and Biology
This part is repressed by BBa_K3320019 and K3320016 in absence of Lactate and Hypoxia, physiological characteristics from the tumor microenvironment. When induced, it expresses the BBa_K3320013 to be secreted directly from the cytosol into the extracellular environment by the alpha-hemolysin secretion system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]