Difference between revisions of "Part:BBa K2999004"
 
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===Usage and Biology===
 
===Usage and Biology===
  
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In ''Pseudomonas aeruginosa'' PAO1, BHL signal molecules need to form a complex with rhlr to start the downstream gene transcription. We knock out genes related to BHL signal molecules and PQS signal molecules, and find that when BHL signal is missing, PA2069 promoter loses its β-Galactosidase activity, that is, PA2069 is the promoter responding to BHL.
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BHL and RhlR are self-inducing signaling molecules and receptor pairs for the <i>P. aeruginosa</i> Quorum sensing. When BHL and RhlR form a complex, they can induce downstream gene transcription.When our team knocks out rhlR gene and rhlI gene separately, <i>PA2069</i> promoter The enzyme activity was significantly reduced, so the <i>PA2069</i> promoter was transformed in response to BHL-RhlR.
  
 
[[File:T--YAU-China--iGEM2019-BBa -6.png|500px|thumb|none|Figure 1.Determination of β - galactosidase activity of PA2069 promoter ]]
 
[[File:T--YAU-China--iGEM2019-BBa -6.png|500px|thumb|none|Figure 1.Determination of β - galactosidase activity of PA2069 promoter ]]

Latest revision as of 17:22, 21 October 2019


PA2069 Promoter

This part is a promoter which is regulated by the quorum sensing system in Pseudomonas aeruginosa, and the signal molecule which regulates the promoter is N-(butyry1)-1-homoserine lactone (BHL).


Usage and Biology

BHL and RhlR are self-inducing signaling molecules and receptor pairs for the P. aeruginosa Quorum sensing. When BHL and RhlR form a complex, they can induce downstream gene transcription.When our team knocks out rhlR gene and rhlI gene separately, PA2069 promoter The enzyme activity was significantly reduced, so the PA2069 promoter was transformed in response to BHL-RhlR.

Figure 1.Determination of β - galactosidase activity of PA2069 promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 365
    Illegal XhoI site found at 698
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 96
    Illegal NgoMIV site found at 271
    Illegal NgoMIV site found at 297
    Illegal NgoMIV site found at 482
    Illegal NgoMIV site found at 602
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 989