Difference between revisions of "Part:BBa K2940001"
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Revision as of 17:21, 21 October 2019
PelB and Bpul
The PelB leader sequence was joined to Bpul laccase to enable exportation of the enzyme into the periplasmic space. Both parts were isolated from the iGEM kit via Q5 PCR and joined together using Gibson Assembly. Not only was exporting Bpul outside of the cell vital for our overall project, it also allowed us to assess the activity of Bpul without the need for expensive and time consuming purification steps.
Usage and Biology
This biobrick was produced to be an integral part of our overall azo dye bioremediation strategy. The joining of the leader sequence PelB allows for the secretion of Bpul into the periplasmic space. PelB directs the unfolded fusion protein to the periplasm, where the peptide is cleaved by a signal peptidase. This causes the protein of interest (here Bpul) to be secreted into the periplasmic space.
Characterisation
