Difference between revisions of "Part:BBa K3285700"
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The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor <a href="https://parts.igem.org/Part:BBa_K1758333">BBa_K1758333</a>. This part has been synthesised with a strong Ribosome Binding Site <a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a> upstream of the coding sequence. | The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor <a href="https://parts.igem.org/Part:BBa_K1758333">BBa_K1758333</a>. This part has been synthesised with a strong Ribosome Binding Site <a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a> upstream of the coding sequence. |
Revision as of 17:17, 21 October 2019
Lead Sequestration Protein with RBS
pbrD is a protein that sequesters lead. It is present downstream of a strong RBS (BBa_B0034) and binds to lead ions in the cytoplasm and decrease its concentration.
Usage and Biology
This is an intermediate part which can be incorporated under a promoter. The pbrD protein is known to sequester lead ions in the cytoplasm of the bacterial cell.
When put under a lead responsive promoter, the part is expected to show dosage dependent sequestration properties.
Construction
File:Https://2019.igem.org/wiki/images/d/d2/T--IISER-Pune-India--pbrD Gel.jpeg |
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The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor <a href="https://parts.igem.org/Part:BBa_K1758333">BBa_K1758333</a>. This part has been synthesised with a strong Ribosome Binding Site <a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a> upstream of the coding sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 532
- 1000COMPATIBLE WITH RFC[1000]