Difference between revisions of "Part:BBa K3100001"

 
 
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<partinfo>BBa_K3100001 short</partinfo>
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<partinfo>BBa_K3100001 short</partinfo><br>
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PT7 is the most well-known inducible promoter with high transcriptional strength. T7 RNAP is a single-subunit RNA polymerase that is a strong driver of transcription. It is functionally orthogonal to most hosts, acting only on its cognate promoter, PT7.<br>
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From the research of Ghodasara A et al, we found different strength of PT7 and made full use of them for our VerProS pool. How our VerProS pool work you can learn from it: https://2019.igem.org/Team:SCUT_China/Design
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<center>[[File:T - SCUT China- T7 promoter 1-48.jpeg|500px]]<br>
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Figure. The strength of PT7 1-48</center>
  
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T7 RNA polimerase can be used in E.coli K12 Mg1655 and transcription from T7 promoter.T7 RNA polimerase can be used in E.coli K12 Mg1655 and transcription from T7 promoter.T7 RNA polimerase can be used in E.coli K12 Mg1655 and transcription from T7 promoter.T7 RNA polimerase can be used in E.coli K12 Mg1655 and transcription from T7 promoter.
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<h3><strong>References:</strong></h3>
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Ghodasara A, Voigt CA. Balancing gene expression without library construction via a reusable sRNA pool. Nucleic Acids Res. 2017;45(13):8116–8127. doi:10.1093/nar/gkx530
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 17:16, 21 October 2019


T7 Promoter T7-1
PT7 is the most well-known inducible promoter with high transcriptional strength. T7 RNAP is a single-subunit RNA polymerase that is a strong driver of transcription. It is functionally orthogonal to most hosts, acting only on its cognate promoter, PT7.
From the research of Ghodasara A et al, we found different strength of PT7 and made full use of them for our VerProS pool. How our VerProS pool work you can learn from it: https://2019.igem.org/Team:SCUT_China/Design

T - SCUT China- T7 promoter 1-48.jpeg
Figure. The strength of PT7 1-48

References:

Ghodasara A, Voigt CA. Balancing gene expression without library construction via a reusable sRNA pool. Nucleic Acids Res. 2017;45(13):8116–8127. doi:10.1093/nar/gkx530

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]