Difference between revisions of "Part:BBa K3110034"

 
 
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<partinfo>BBa_K3110034 short</partinfo>
 
<partinfo>BBa_K3110034 short</partinfo>
  
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L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) and the regulator element (lldR) under the control of a medium promoter and a strong RBS.
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This construct has L-Lactate Permease (lldP), Regulator element (lldR) and L-Lactate Dehydrogenase (lldD) under the control of a medium promoter and strong RBS . lldP permits the entry of L-Lactate inside the bacterial cell, lldR has the dual role of an activator and a repressor and  lldD cleaves L-Lactate to pyruvate .  Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldP, lldR and lldD by varying  the strength of the promoter and RBS controlling their production.
  
  
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<partinfo>BBa_K3110034 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3110034 SequenceAndFeatures</partinfo>
  
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===Characterisation===
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The construct could not be characterised due to insufficient time.
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3110034 parameters</partinfo>
 
<partinfo>BBa_K3110034 parameters</partinfo>
 
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Latest revision as of 17:12, 21 October 2019


Medium Promoter Strong RBS lldP+lldR+lldD

This construct has L-Lactate Permease (lldP), Regulator element (lldR) and L-Lactate Dehydrogenase (lldD) under the control of a medium promoter and strong RBS . lldP permits the entry of L-Lactate inside the bacterial cell, lldR has the dual role of an activator and a repressor and lldD cleaves L-Lactate to pyruvate . Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldP, lldR and lldD by varying the strength of the promoter and RBS controlling their production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3650
    Illegal AgeI site found at 464
    Illegal AgeI site found at 927
    Illegal AgeI site found at 1365
    Illegal AgeI site found at 1524
    Illegal AgeI site found at 1536
    Illegal AgeI site found at 2282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3087

Characterisation

The construct could not be characterised due to insufficient time.