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Latest revision as of 17:11, 21 October 2019
Tobacco Vein Mottling Virus Protease Cleavage Site
Profile
| Name | Tobacco Vein Mottling Virus (TVMV) Protease Cleavage Site |
| Base pairs | 18 |
| Origin | Tobacco Vein Mottling Virus (TVMV) |
| Properties | recognition site for the TVMV protease |
Usage and Biology
The protease cleavage site from the Tobacco Vein Mottling Virus (TVMV) can be used to specifically cleave a protein at the sequence ETVRFQS.
We used this recognition site to cleave our protein in front of its polyG-tag, so that it has a C-terminal polyG-tag that can be used for sortase-mediated ligation of a GGGG-tagged and a LPETGG-tagged protein.
To read more about the sortase-mediated ligation, please have a look at our wiki.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]