Difference between revisions of "Part:BBa K3187020"

 
(10 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3187020 short</partinfo>
 
<partinfo>BBa_K3187020 short</partinfo>
 +
<html>
  
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.
+
                <h3>Profile</h3>
 +
                <table style=“width:80%“>
 +
                <tr>
 +
                <td><b>Name</b></td>
 +
                <td>Tobacco Vein Mottling Virus (TVMV) Protease Cleavage Site</td>
 +
                </tr>
 +
 
 +
                <tr>
 +
                <td><b>Base pairs</b></td>
 +
                <td>18</td>
 +
                </tr>
 +
 
 +
           
 +
                <tr>
 +
                <td><b>Origin</b></td>
 +
                <td>Tobacco Vein Mottling Virus (TVMV) </td>
 +
                </tr>
 +
 
 +
                <tr>
 +
                <td><b>Properties</b></td>
 +
                <td> recognition site for the TVMV protease</td>
 +
                </tr>
 +
                </table>
 +
</html>
  
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
<html>               
 +
 +
                <p>
 +
The protease cleavage site from the Tobacco Vein Mottling Virus (TVMV) can be used to specifically cleave a protein at the sequence ETVRFQS.
 +
</p>
 +
<p>
 +
We used this recognition site to cleave our protein in front of its polyG-tag, so that it has a C-terminal polyG-tag that can be used for sortase-mediated ligation of a GGGG-tagged and a LPETGG-tagged protein.
 +
<br><br>
 +
To read more about the sortase-mediated ligation, please have a look at our <a href=https://2019.igem.org/Team:TU_Darmstadt/Project/Sortase>wiki</a>.
 +
</p>
 +
</html>
 +
  
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3187020 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3187020 SequenceAndFeatures</partinfo>
The TVMV protease cuts after the final Gln. Cleavage works best if the following amino acid is Gly or Ser, but other amino acids at P1' except proline <a href="http://onlinelibrary.wiley.com/doi/full/10.1002/pro.506" target="_blank">work as well</a>
 
  
<!-- Uncomment this to enable Functional Parameter display
+
 
===Functional Parameters===
+
<!-- ===Functional Parameters=== -->
 
<partinfo>BBa_K3187020 parameters</partinfo>
 
<partinfo>BBa_K3187020 parameters</partinfo>
<!-- -->
 

Latest revision as of 17:11, 21 October 2019


Tobacco Vein Mottling Virus Protease Cleavage Site

Profile

Name Tobacco Vein Mottling Virus (TVMV) Protease Cleavage Site
Base pairs 18
Origin Tobacco Vein Mottling Virus (TVMV)
Properties recognition site for the TVMV protease

Usage and Biology

The protease cleavage site from the Tobacco Vein Mottling Virus (TVMV) can be used to specifically cleave a protein at the sequence ETVRFQS.

We used this recognition site to cleave our protein in front of its polyG-tag, so that it has a C-terminal polyG-tag that can be used for sortase-mediated ligation of a GGGG-tagged and a LPETGG-tagged protein.

To read more about the sortase-mediated ligation, please have a look at our wiki.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]