Difference between revisions of "Part:BBa K3187020"

Revision as of 17:09, 21 October 2019

Tobacco Vein Mottling Virus Protease Cleavage Site

Profile

Name	Tobacco Vein Mottling Virus (TVMV) Protease Cleavage Site
Base pairs	18
Origin	Tobacco Vein Mottling Virus (TVMV)
Properties	recognition site for the TVMV protease

Usage and Biology

The protease cleavage site from the Tobacco Vein Mottling Virus (TVMV) can be used to specifically cleave a protein at the sequence ETVRFQS.

We used this recognition site to cleave our protein in front of its polyG-tag, so that it has a C-terminal polyG-tag that can be used for sortase-mediated ligation of a GGGG-tagged and a LPETGG-tagged protein.

To read more about the sortase-mediated ligation, please have a look at our wiki.

Test

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Uncomment this to enable Functional Parameter display

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 Test
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 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3187020 SequenceAndFeatures</partinfo>
-The TVMV protease cuts after the final Gln. Cleavage works best if the following amino acid is Gly or Ser, but other amino acids at P1' except proline <a href="http://onlinelibrary.wiley.com/doi/full/10.1002/pro.506" target="_blank">work as well</a>
-<!-- Uncomment this to enable Functional Parameter display
+ Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K3187020 parameters</partinfo>
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