Difference between revisions of "Part:BBa K592002"
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ccaR is the response regulator of the green sensor (BBa_K592001) that also includes the membrane-associated histidine kinase ccaS. The two-component system is also dependent of a chromophore.  
 
ccaR is the response regulator of the green sensor (BBa_K592001) that also includes the membrane-associated histidine kinase ccaS. The two-component system is also dependent of a chromophore.  
  
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===Usage and Biology===
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<img src=“https://2019.igem.org/wiki/images/9/96/T--NYU_New_York--results1.png“><br>
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<p>Figure 1: Plates containing the pSR58.6 (left) and pNO286-3 (right) plasmids under UV light. The pSR58.6 plasmid contains CcaR and superfolder GFP. The pNO286-3 plasmid contains the mini-CcaS gene, along with ho1 and pcyA.</p>
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<img src=“https://2019.igem.org/wiki/images/a/a3/T--NYU_New_York--results2.jpg”><br>
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<p>Figure 2: Plate containing cells co-transformed with both pSR58.6 and pNO286-3 plasmids.</p>
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<img src=“https://2019.igem.org/wiki/images/6/60/T--NYU_New_York--results3.png”><br>
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<p>Figure 3: Light sensor readings from plates containing optogenetic plasmids</p>
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Revision as of 17:09, 21 October 2019

ccaR

ccaR is the response regulator of the green sensor (BBa_K592001) that also includes the membrane-associated histidine kinase ccaS. The two-component system is also dependent of a chromophore.


<img src=“https://2019.igem.org/wiki/images/9/96/T--NYU_New_York--results1.png“>

Figure 1: Plates containing the pSR58.6 (left) and pNO286-3 (right) plasmids under UV light. The pSR58.6 plasmid contains CcaR and superfolder GFP. The pNO286-3 plasmid contains the mini-CcaS gene, along with ho1 and pcyA.


<img src=“https://2019.igem.org/wiki/images/a/a3/T--NYU_New_York--results2.jpg”>

Figure 2: Plate containing cells co-transformed with both pSR58.6 and pNO286-3 plasmids.


<img src=“https://2019.igem.org/wiki/images/6/60/T--NYU_New_York--results3.png”>

Figure 3: Light sensor readings from plates containing optogenetic plasmids


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 402
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]