Difference between revisions of "Part:BBa K2972001:Design"

 
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===Design Notes===
 
===Design Notes===
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The two ends of this sequence are highly similar to sigmaB. It may lead to the failure of PCR.
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Make sure it is orthogonal to the host cell before use.
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===Source===
 
===Source===
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Sigma F is derived from Strains B. subtilis subsp. subtilis wild-type (LMG 7135), and it’s a regulatory protein that is responsible for directing gene expression in the forespore compartment of developing cells of the spore-forming soil bacterium Bacillus subtilis.
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It come from Bacillus subtilis genomic sequence.
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===References===
 
===References===

Latest revision as of 17:06, 21 October 2019


Sigma F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

Make sure it is orthogonal to the host cell before use.

Source

Sigma F is derived from Strains B. subtilis subsp. subtilis wild-type (LMG 7135), and it’s a regulatory protein that is responsible for directing gene expression in the forespore compartment of developing cells of the spore-forming soil bacterium Bacillus subtilis.

References