Difference between revisions of "Part:BBa K3182009"

Latest revision as of 17:02, 21 October 2019

Parseq-α

Parseq Alpha is a de novo antimicrobial peptide. It was generated by the Parseq model in combination with an evolution script. This part was first ordered chemically synthesized by Caslo to investigate its antimicrobial activity on its own. The peptides were dissolved in 6 M Urea due to their hydrophobic nature. The activity was tested on E.coli and B.Subtilis. The antimicrobial activity assay was carried out in a 96 well plate. Bacteria were combined with the antimicrobial peptides in different concentrations. They were then incubated at 37°C, with shaking, every 15 minutes. The plate was incubated for 16 hours and OD₆₀₀ was measured. The results can be seen in Figure 1. Parseq Alpha showed an inhibiting effect on the growth of E.coli. A concentration of 30 µg/mL completely inhibited the growth of E.coli. In comparison, B.subtilis could not be inhibited completely by Parseq Alpha. However, high concentrations of the peptide showed partial inhibition of the growth of B.subtilis, where the lag phase is extended.

Figure 1 Shows the antimicrobial activity of Parseq Alpha on A) E.Coli. and B) B.subtilis. The samples were cultured in 200 µL low salt LB, in a 96 well plate and incubated at 37°C. Shaking was conducted for 1 minute every 15 minutes and the OD₆₀₀ was measured every hour for 16 hours. The negative control represents the bacterial growth with no antimicrobial peptides present but the same buffer the peptides were dissolved in. All measurements are done in 5 replicates and error bars represent SD. The graphs were created in Graphpad.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3182009 short</partinfo>
-Parseq alpha is a de novo antimicrobial peptide. It was generated by the Parseq model and the antimicrobial activity was predicted by the Parseq model. This part was designed to be assembled with BBa_K3182010. This part was first ordered chemically synthesised by Caslo to investigate its antimicrobial activity on its own. The peptides were dissolved in 6 M Urea do to there hydrophobic nature. The activity was tested on E.coli and B.Subtilis. The antimicrobial activity assay was carried out in a 96 well plate. Bacteria were combined with the antimicrobial peptide in different concentration. they were then incubated at 37°C with shaking every 15 minutes. the plate was incubated for 16 hours and OD<sup>600</sup> was measured. The results from this can be seen in <b>Figure 1.</b>. Parseq alpha showed an inhibiting effect on the growth of E.coli. 30 ng/mL completely inhibited the growth of E.coli. B.subtilis could not be inhibited completely by Parseq Alpha. However, high concentrations of the peptide showed partial inhibition of the growth of B.subtilis.
+Parseq Alpha is a de novo antimicrobial peptide. It was generated by the Parseq model in combination with an evolution script.  This part was first ordered chemically synthesized by Caslo to investigate its antimicrobial activity on its own. The peptides were dissolved in 6 M Urea due to their hydrophobic nature. The activity was tested on <i>E.coli</i> and <i>B.Subtilis</i>. The antimicrobial activity assay was carried out in a 96 well plate. Bacteria were combined with the antimicrobial peptides in different concentrations. They were then incubated at 37°C, with shaking, every 15 minutes. The plate was incubated for 16 hours and OD<sub>600</sub> was measured. The results can be seen in <b>Figure 1.</b> Parseq Alpha showed an inhibiting effect on the growth of <i>E.coli</i>. A concentration of 30 µg/mL completely inhibited the growth of <i>E.coli</i>. In comparison, <i>B.subtilis</i> could not be inhibited completely by Parseq Alpha. However, high concentrations of the peptide showed partial inhibition of the growth of <i>B.subtilis</i>, where the lag phase is extended.
 [[File:T--Linkoping_Sweden--AlphaEcoli.png|650px|thumb|center|<b>Figure 1</b> Shows the antimicrobial activity of Parseq Alpha
-  on A) <i>E.Coli.</i> and B) <i>B.subtilis</i>. The samples were cultured in 200 µL in a 96 well plate and incubated at 37°C. shaking was conducted for 1 minute every 15 minutes and the OD<sup>600</sup> were messured every hour for 16 hours. the negative control represents the bacterial growth with no antimicrobial peptides precent. All measurements are done in 6 replicates and error bars represent SD. The graphs were created in Graphpad ]]
+  on A) <i>E.Coli.</i> and B) <i>B.subtilis</i>. The samples were cultured in 200 µL low salt LB, in a 96 well plate and incubated at 37°C. Shaking was conducted for 1 minute every 15 minutes and the OD<sub>600</sub> was measured every hour for 16 hours. The negative control represents the bacterial growth with no antimicrobial peptides present but the same buffer the peptides were dissolved in. All measurements are done in 5 replicates and error bars represent SD. The graphs were created in Graphpad.]]