Difference between revisions of "Part:BBa K3179105"

Latest revision as of 17:00, 21 October 2019

pT2mKE

pT2mKE(pTRE-mi663b-2Kt-E1A-miR885-5p)

Usage and Biology

This part consists of miR663b-target, 2kt-E1A and miR885-5p-target,which be put in the vector pTRE. From promoter CMV our plasmid can transcribe mRNA with miR663b-target, 2kt-E1A and miR885-5p-target, whose translation can be regulated by level of free miRNA663b and miRNA885-5p, and also by L7Ae. When they exist, the translation of this mRNA will be suppressed. The production is E1A, which can start the expression of adenoviral gene. Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 999
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 203
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 999
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 999
Illegal NgoMIV site found at 473
Illegal AgeI site found at 209
1000
COMPATIBLE WITH RFC[1000]

Revision as of 20:31, 20 October 2019 (view source) Bigxushen (Talk \| contribs) ← Older edit		Latest revision as of 17:00, 21 October 2019 (view source) Maggie-WZE (Talk \| contribs)
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	<partinfo>BBa_K3179105 short</partinfo>		<partinfo>BBa_K3179105 short</partinfo>

−	pT2mKE(pTRE-mi663b-2Kt-E1-miR885-5p)	+	pT2mKE(pTRE-mi663b-2Kt-E1A-miR885-5p)