Difference between revisions of "Part:BBa K3229290"

 
 
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Microcystis aeruginosa NIES:843 strain 3505068-3505974.
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This is a putative regulatory DNA which probably contains the promoters for microcystin toxin-producing enzymes in two directions: mcyABC and mcyDEFGHIJ.
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This is the putative promoter of microcystin toxin-producing proteins from Microcysis aeruginosa NIES-843. We looked up the promoter sequence in Ensemble Bacteria (http://bacteria.ensembl.org). We found many sequences that differ between strains, by the help of Geneious Prime we found the strain NIES-843 which has the most conservative sequence based on the consensus sequence we got. From the Microcystis promoter transcription can occur in both directions to synthesize the McyABC and McyDEFGHIJ proteins. These proteins are responsible for the nonribosomal peptide synthesis of the toxin, called microcystin. The presence of this toxin enhances the production of toxins in other cells via the phenomenon called autoinduction. For this reason, we could use this promoter in our microcystin detecting device.
  
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https://static.igem.org/mediawiki/parts/7/73/T--SZTA_Szeged_HU--Originalsequence.png
 
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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Latest revision as of 16:59, 21 October 2019


McyA/D bidirectional promoter

This is the putative promoter of microcystin toxin-producing proteins from Microcysis aeruginosa NIES-843. We looked up the promoter sequence in Ensemble Bacteria (http://bacteria.ensembl.org). We found many sequences that differ between strains, by the help of Geneious Prime we found the strain NIES-843 which has the most conservative sequence based on the consensus sequence we got. From the Microcystis promoter transcription can occur in both directions to synthesize the McyABC and McyDEFGHIJ proteins. These proteins are responsible for the nonribosomal peptide synthesis of the toxin, called microcystin. The presence of this toxin enhances the production of toxins in other cells via the phenomenon called autoinduction. For this reason, we could use this promoter in our microcystin detecting device.

T--SZTA_Szeged_HU--Originalsequence.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]