Difference between revisions of "Part:BBa K2972004:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | ... | + | The identification of this gene in E. coli prokaryotic expression system is currently mainly accomplished by heterologous complementation detection. |
− | + | When designing primers, it is recommended that the first 6 bps of the 5' end cannot form a card issuance structure. | |
− | + | ||
===Source=== | ===Source=== | ||
− | + | The sequence of this part is obtained from NCBI and it comes from an E.coli's genome which intergrated lycopene gene. | |
===References=== | ===References=== |
Latest revision as of 16:26, 21 October 2019
Phytoene desaturase (crtI)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 696
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 154
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 742
Illegal SapI.rc site found at 190
Design Notes
The identification of this gene in E. coli prokaryotic expression system is currently mainly accomplished by heterologous complementation detection. When designing primers, it is recommended that the first 6 bps of the 5' end cannot form a card issuance structure.
Source
The sequence of this part is obtained from NCBI and it comes from an E.coli's genome which intergrated lycopene gene.