Difference between revisions of "Part:BBa K2940001"
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<partinfo>BBa_K2940001 short</partinfo>
 
<partinfo>BBa_K2940001 short</partinfo>
  
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The PelB leader sequence was joined to Bpul laccase to enable exportation of the enzyme into the periplasmic space.   
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The PelB leader sequence was joined to Bpul laccase to enable exportation of the enzyme into the periplasmic space.  Both parts were isolated from the iGEM kit via Q5 PCR and joined together using Gibson Assembly.
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<h2>Usage and Biology</h2>
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<b> Cultivation and SDS Page </b>
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2940001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2940001 SequenceAndFeatures</partinfo>
  
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[[File:T--Edinburgh_OG--BBa_K2940001-SDS_image1.jpg|200px|thumb|left|alt text]]
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 16:19, 21 October 2019


PelB and Bpul

The PelB leader sequence was joined to Bpul laccase to enable exportation of the enzyme into the periplasmic space. Both parts were isolated from the iGEM kit via Q5 PCR and joined together using Gibson Assembly.

Usage and Biology

Cultivation and SDS Page


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 1030
    Illegal AgeI site found at 318
    Illegal AgeI site found at 1195
  • 1000
    COMPATIBLE WITH RFC[1000]
File:T--Edinburgh OG--BBa K2940001-SDS image1.jpg
alt text