Difference between revisions of "Part:BBa K143006"

Latest revision as of 22:58, 25 October 2008

3' Integration Sequence for the epsE locus of B. subtilis

Integration sequences allow DNA to be incorporated into the chromosome of a host cell at a specific locus using leading (5') and trailing (3') DNA sequences that are the same as those at a specific locus of the chromosome. The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (BBa_K143005) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B. subtilis at the epsE locus.

The epsE (aka YveO) locus has to our knowledge never been used for integration into B. subtilis before but is useful as it knocks out the potential molecular clutch epsE gene #1. In particular, both the 5' and 3' integration sequences for the EpsE locus conatin in-frame stop codons to prevent translation of the gene (if nothing is integrated into the locus, integration also prevents correct epsE expression). The 5' and 3' integration sequences for the epsE locus were used to integrate over the epsE gene and prevent its expression in the Imperial 2008 iGEM project B. subtilis host.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 211
1000
COMPATIBLE WITH RFC[1000]

References

1 pmid=18566286

</biblio>

@@ Line 1: / Line 1: @@
 __NOTOC__
+===<big>3' Integration Sequence for the epsE locus of ''B. subtilis''</big>===
-===<big>3' Integration Sequence for the EpsE locus of B. subtilis</big>===
 [[Image:EpsE_Integration.png|center|800px]]
-Integration sequences allow DNA to be incorporated into the chromosome of a host cell at a specific locus using leading (5') and trailing (3') DNA sequences that are the same as those at a specific locus of the chromosome. The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (<bbpart>BBa_K143005</bbpart>) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B.subtilis.
+Integration sequences allow DNA to be incorporated into the chromosome of a host cell at a specific locus using leading (5') and trailing (3') DNA sequences that are the same as those at a specific locus of the chromosome. The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (<bbpart>BBa_K143005</bbpart>) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium ''B. subtilis'' at the epsE locus.
+The epsE (aka YveO) locus has to our knowledge never been used for integration into ''B. subtilis'' before but is useful as it knocks out the potential molecular clutch epsE gene <cite>#1</cite>. In particular, both the 5' and 3' integration sequences for the EpsE locus conatin in-frame stop codons to prevent translation of the gene (if nothing is integrated into the locus, integration also prevents correct epsE expression). The 5' and 3' integration sequences for the epsE locus were used to integrate over the epsE gene and prevent its expression in the Imperial 2008 iGEM project ''B. subtilis'' host.
+<!-- -->
+<span class='h3bb'><big>'''Sequence and Features'''</big></span>
-The EpsE (aka YveO) locus has to our knowledge never been used for integration into ''B.subtilis'' before, but is useful in that it knocks out the potential molecular clutch EpsE gene <cite>#1</cite>. In particular, both the 5' and 3' integration sequences for the EpsE locus conatin in-frame stop codons to prevent translation of the gene (if nothing is integrated into the locus, integration also prevents correct EpsE expression). The 5' and 3' integration sequences for the EpsE locus were used to integrate over the EpsE gene and prevent its expression in the Imperial 2008 iGEM project ''B.sutbilis'' host.
+<partinfo>BBa_K143006 SequenceAndFeatures</partinfo>
 ===References===