Difference between revisions of "Part:BBa K3081063"
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"This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20bp sequence targeting the IHF binding region of plasmid replication origin pSC101,using in the CRISPRri system<partinfo>BBa_K3081007</partinfo> to control the plasmid copy number." | "This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20bp sequence targeting the IHF binding region of plasmid replication origin pSC101,using in the CRISPRri system<partinfo>BBa_K3081007</partinfo> to control the plasmid copy number." | ||
+ | <center> | ||
+ | https://2019.igem.org/wiki/images/c/cf/T--Peking--plasmid_copy_number_pL01.png | ||
+ | </center> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 16:18, 21 October 2019
pBAD-dCas9-J23119-pL05
"This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20bp sequence targeting the IHF binding region of plasmid replication origin pSC101,using in the CRISPRri systemBBa_K3081007 to control the plasmid copy number."
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 5459
Illegal NheI site found at 5482 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1470
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961