Difference between revisions of "Part:BBa K143001:Design"
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− | ===<big>'''5' Integration sequence for the | + | ===<big>'''5' Integration sequence for the amyE locus of ''B. subtilis'''''</big>=== |
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===Design Notes=== | ===Design Notes=== | ||
− | The | + | The amyE integration sequence was taken from the vector after comparison by BLAST to the ''B. subtilis'' chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 5' end of the gene is the 5' sequence required for integration |
===Source=== | ===Source=== |
Revision as of 22:55, 25 October 2008
5' Integration sequence for the amyE locus of B. subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The amyE integration sequence was taken from the vector after comparison by BLAST to the B. subtilis chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 5' end of the gene is the 5' sequence required for integration
Source
The 5’ integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on B. subtilis, in particular in the studies of transcriptional control#1,#2,#3
References
<biblio>
- 1 pmid=14597697
- 2 pmid=15937167
- 3 pmid=12169614
</biblio>