Difference between revisions of "Part:BBa K3040001"
(One intermediate revision by the same user not shown) | |||
Line 3: | Line 3: | ||
<partinfo>BBa_K3040001 short</partinfo> | <partinfo>BBa_K3040001 short</partinfo> | ||
− | Lipase A1 from Antarctic deep sea bacterium, Psychrobacter sp 7195., can catalyze the hydrolysis of | + | Lipase A1 from Antarctic deep-sea bacterium, Psychrobacter sp 7195., can catalyze the hydrolysis of triacylglycerols to glycerol and monoacyl-glycerols. We utilize the characteristic of lipase-having different catalytic activity under different temperature, to sense the temperature change. Lipase A1 reaches its catalytic activity peak at about 30℃, and decreases as temperature rise or drops. Furthermore, to purify our lipase A in an easier way, we design a His-tag sequence behind the lipase A. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 16:14, 21 October 2019
Lipase A1 (Psychrobacter sp 7195.) -Histag
Lipase A1 from Antarctic deep-sea bacterium, Psychrobacter sp 7195., can catalyze the hydrolysis of triacylglycerols to glycerol and monoacyl-glycerols. We utilize the characteristic of lipase-having different catalytic activity under different temperature, to sense the temperature change. Lipase A1 reaches its catalytic activity peak at about 30℃, and decreases as temperature rise or drops. Furthermore, to purify our lipase A in an easier way, we design a His-tag sequence behind the lipase A.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1186