Difference between revisions of "Part:BBa K3183000"

(→‎Characterisation)
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===Use by Team Oxford 2019===
 
===Use by Team Oxford 2019===
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This transcription factor is part of our detection system in <i>L. reuteri</i>. The composite parts containing this AgrA2 protein are <partinfo>BBa_K3183102</partinfo>, and <partinfo>BBa_K3183300</partinfo>.
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This promoter was used in number of composite parts by Team Oxford 2019. The composite parts containing P-erm are <partinfo>BBa_K3183103</partinfo>, <partinfo>BBa_K3183100</partinfo>, and <partinfo> BBa_K3183028</partinfo>.
  
  
 
===Characterisation===
 
===Characterisation===
  
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This part was characterised in the composite part [https://parts.igem.org/Part:BBa_K3183100 BBa_K3183100.]
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This part was characterised in the composite parts <partinfo>BBa_K3183100</partinfo>, and <partinfo> BBa_K3183028</partinfo> .
  
 
===References===
 
===References===
 
1. Swinfield, Tracy-Jane, et al. “Physical Characterisation of the Replication Region of the Streptococcus Faecalis Plasmid pAMβ1.” Gene, vol. 87, no. 1, 1990, pp. 79–90., doi:10.1016/s0378-1119(19)30488-3. <br>
 
1. Swinfield, Tracy-Jane, et al. “Physical Characterisation of the Replication Region of the Streptococcus Faecalis Plasmid pAMβ1.” Gene, vol. 87, no. 1, 1990, pp. 79–90., doi:10.1016/s0378-1119(19)30488-3. <br>
 
2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri  Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.
 
2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri  Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.

Revision as of 16:11, 21 October 2019


Erythromycin Constitutive Promoter

P-erm is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species. It has also been shown to be functional in E. coli.

The promoter is derived from the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAMβ1 isolated from Enterococcus faecalis.1 It was subsequently characterised in six strains of Lactobacillus reuteri and Lactococcus lactisspp. cremoris MG1363.2


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Use by Team Oxford 2019

This promoter was used in number of composite parts by Team Oxford 2019. The composite parts containing P-erm are BBa_K3183103, BBa_K3183100, and No part name specified with partinfo tag..


Characterisation

This part was characterised in the composite parts BBa_K3183100, and No part name specified with partinfo tag. .

References

1. Swinfield, Tracy-Jane, et al. “Physical Characterisation of the Replication Region of the Streptococcus Faecalis Plasmid pAMβ1.” Gene, vol. 87, no. 1, 1990, pp. 79–90., doi:10.1016/s0378-1119(19)30488-3.
2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri  Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.