Difference between revisions of "Part:BBa K3286100:Design"
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===References===
 
===References===
  
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[1] V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.
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<li>[1] V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.</li>
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[2] Nieuwkoop, T., Claassens, N. J., & van der Oost, J. (2019). Improved protein production and codon optimization analyses in Escherichia coli by bicistronic design. Microbial biotechnology, 12(1), 173-179.
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<li>[2] Nieuwkoop, T., Claassens, N. J., & van der Oost, J. (2019). Improved protein production and codon optimization analyses in Escherichia coli by bicistronic design. Microbial biotechnology, 12(1), 173-179.</li>

Latest revision as of 16:10, 21 October 2019


Expression construct for proteins in E. coli BL21DE3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Strep tag on the N-terminus, with the TEV cleavage site in between, which can be cut using TEV protease. This will yield the recombinant protein.


Source

Parts amplified from plasmid present at our university

References

  • [1] V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.
  • [2] Nieuwkoop, T., Claassens, N. J., & van der Oost, J. (2019). Improved protein production and codon optimization analyses in Escherichia coli by bicistronic design. Microbial biotechnology, 12(1), 173-179.