Difference between revisions of "Part:BBa K3110044"

 
 
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<partinfo>BBa_K3110044 short</partinfo>
 
<partinfo>BBa_K3110044 short</partinfo>
  
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This part is a control for its lactate inducible counterpart <a href="https://parts.igem.org/Part:BBa_K3110041">.
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There is constitutive Promoter (J23117) followed by Medium RBS (B0032) and sfGFp (reporter protein). This part can be used as a control for lldRO1-J23117-lldRO2 Medium RBS sfGFP (BBa_K3110041) to find out the effect of the operator sequences (lldRO1 and lldRO2) in presence of Lactate rich environment (Tumor Micro-environment).
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K3110044 parameters</partinfo>
 
<partinfo>BBa_K3110044 parameters</partinfo>
 
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<h1>Characterization </h1>
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Due to shortage of time we could not characterize this construct.

Latest revision as of 15:59, 21 October 2019


J23117 Medium RBS sfGFP

There is constitutive Promoter (J23117) followed by Medium RBS (B0032) and sfGFp (reporter protein). This part can be used as a control for lldRO1-J23117-lldRO2 Medium RBS sfGFP (BBa_K3110041) to find out the effect of the operator sequences (lldRO1 and lldRO2) in presence of Lactate rich environment (Tumor Micro-environment).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 67


Characterization

Due to shortage of time we could not characterize this construct.