Difference between revisions of "Part:BBa K3110041"

 
 
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lldRO1 and lldRO2 are the operator regions which bind to the regulator element lldR and inhibit transcription. Upon binding of L-Lactate to lldR, however, this transcriptional suppression is lost and instead lldR complex with L-Lactate remains bound to lldRO1 acting as a transcriptional activator. By using RBS of different strengths we are trying to find out the optimum construct which will give output (in our case the output is super-folded GFP or sfGFP) that can efficiently discriminate cancer vs non-cancer microenvironments.
 
lldRO1 and lldRO2 are the operator regions which bind to the regulator element lldR and inhibit transcription. Upon binding of L-Lactate to lldR, however, this transcriptional suppression is lost and instead lldR complex with L-Lactate remains bound to lldRO1 acting as a transcriptional activator. By using RBS of different strengths we are trying to find out the optimum construct which will give output (in our case the output is super-folded GFP or sfGFP) that can efficiently discriminate cancer vs non-cancer microenvironments.
  
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K3110041 parameters</partinfo>
 
<partinfo>BBa_K3110041 parameters</partinfo>
 
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<h1>Characterization </h1>
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Due to shortage of time we could not characterize this construct.

Latest revision as of 15:55, 21 October 2019


lldRO1-J23117-lldRO2 Medium RBS sfGFP

lldRO1 and lldRO2 are the operator regions which bind to the regulator element lldR and inhibit transcription. Upon binding of L-Lactate to lldR, however, this transcriptional suppression is lost and instead lldR complex with L-Lactate remains bound to lldRO1 acting as a transcriptional activator. By using RBS of different strengths we are trying to find out the optimum construct which will give output (in our case the output is super-folded GFP or sfGFP) that can efficiently discriminate cancer vs non-cancer microenvironments.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 175


Characterization

Due to shortage of time we could not characterize this construct.