Difference between revisions of "Part:BBa K3132014"

Latest revision as of 15:53, 21 October 2019

4*UAS

4*UAS is 4*Gal4 binding sites from SV40_UAS promoter(fudan2017 Part:BBa_K2446036). In our project, we inset 4*Gal4 binding sites before the minimalCMV promoter. In this way, we can use our synthetic transcription factors (SynTFs) which is a fusing protein Gal4_DBD_(G4S) linker_NLS_VP64, for the Gal4-DBD can bind to the 4*Gal4 binding sites specifically and the VP64 domain can initiate transcription by activating the 4*UAS MinimalCMV promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3132014 short</partinfo>
-*UAS is 4*Gal4 binding sites from SV40_UAS promoter(fudan2017 Part:BBa_K2446036). In our project, we inset 4*Gal4 binding sites before the minimalCMV promoter. In this way, we can use our synthetic transcription factors (SynTFs) which is a fusing protein Gal4_DBD_(G4S) linker_NLS_VP64, for the Gal4-DBD can bind to the 4*Gal4 binding sites specifically and the VP64 domain can initiate transcription by activating the 4*UAS MinimalCMV promoter.
+*UAS is 4*Gal4 binding sites from SV40_UAS promoter(fudan2017 Part:<partinfo>BBa_K2446036</partinfo>). In our project, we inset 4*Gal4 binding sites before the minimalCMV promoter. In this way, we can use our synthetic transcription factors (SynTFs) which is a fusing protein Gal4_DBD_(G4S) linker_NLS_VP64, for the Gal4-DBD can bind to the 4*Gal4 binding sites specifically and the VP64 domain can initiate transcription by activating the 4*UAS MinimalCMV promoter.
 <!-- Add more about the biology of this part here