Difference between revisions of "Part:BBa K2956000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We chose the J23100 Promoter because it is a good one.
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This part is a composite part consisting of BBa_J23100 promoter, BBa_B0034 ribosome binding sequence, and BBa_K2956000 coding region for flavone synthase (FNS). These parts were assembled together to allow for constitutive expression of the FNS gene which converts naringenin to apigenin. When inserted into a vector plasmid and expressed in E. coli, naringenin was shown to be converted at a rate that was positively correlated to cell growth, demonstrating the efficacy of this system.
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===Source===
 
===Source===

Revision as of 15:53, 21 October 2019


RBS-FNS under J23100 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 794
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a composite part consisting of BBa_J23100 promoter, BBa_B0034 ribosome binding sequence, and BBa_K2956000 coding region for flavone synthase (FNS). These parts were assembled together to allow for constitutive expression of the FNS gene which converts naringenin to apigenin. When inserted into a vector plasmid and expressed in E. coli, naringenin was shown to be converted at a rate that was positively correlated to cell growth, demonstrating the efficacy of this system.

Source

This part contains flavone synthase (FNS) from Gerbera hybrida.

References