Difference between revisions of "Part:BBa K3132002"

Latest revision as of 15:50, 21 October 2019

ZF21-16-VP64

Part ZF21-16-VP64 is a change of Part ZF_21-16KRAB (BBa_K2446039). Both parts are mammalian synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 21-16 (ZF_21-16) as protein chassis. However, our part is a change on BBa_K747096 by the replacement of KRAB transcription regulating domain with VP64 transcription regulating domain. So, our part is made up of three core domains from N-terminal to C-terminal: ZF_21-16 DNA binding domain(p58), nuclear location sequence and VP64 transcription regulating domain. The placement of VP64 allows for the activation of expression of our mammalian synthetic promoter CMV-8_ZF_21-16, while KRAB was used to repress the expression of CMV-8_ZF_21-16. At the same time, we changed corresponding mammalian synthetic promoter. When inhibited, the binding sites that KRAB transcription regulating domain specifically binds should be behind the promoter, so the mammalian synthetic promoter CMV-8_ZF_21-16 was before the binding sites. When activated, the binding sites that VP64 transcription regulating domain specifically binds should be before the promoter, so our mammalian synthetic promoter CMV-8_ZF_21-16 was behind the binding sites.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 301
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3132002 short</partinfo>
-Part ZF21-16-VP64 is a change of Part ZF_21-16KRAB (BBa_K2446039). Both parts are mammalian synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 21-16 (ZF_21-16) as protein chassis. However, our part is a change on BBa_K747096 by the replacement of KRAB transcription regulating domain with VP64 transcription regulating domain. So, our part is made up of three core domains from N-terminal to C-terminal: ZF_21-16 DNA binding domain(p58), nuclear location sequence and VP64 transcription regulating domain. The placement of VP64 allows for the activation of expression of our mammalian synthetic promoter CMV-8_ZF_21-16, while KRAB was used to repress the expression of CMV-8_ZF_21-16.
+Part ZF21-16-VP64 is a change of Part ZF_21-16KRAB (<partinfo>BBa_K2446039</partinfo>). Both parts are mammalian synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 21-16 (ZF_21-16) as protein chassis. However, our part is a change on <partinfo>BBa_K747096 </partinfo> by the replacement of KRAB transcription regulating domain with VP64 transcription regulating domain. So, our part is made up of three core domains from N-terminal to C-terminal: ZF_21-16 DNA binding domain(p58), nuclear location sequence and VP64 transcription regulating domain. The placement of VP64 allows for the activation of expression of our mammalian synthetic promoter CMV-8_ZF_21-16, while KRAB was used to repress the expression of CMV-8_ZF_21-16.
 At the same time, we changed corresponding mammalian synthetic promoter. When inhibited, the binding sites that KRAB transcription regulating domain specifically binds should be behind the promoter, so the mammalian synthetic promoter CMV-8_ZF_21-16 was before the binding sites. When activated, the binding sites that VP64 transcription regulating domain specifically binds should be before the promoter, so our mammalian synthetic promoter CMV-8_ZF_21-16 was behind the binding sites.