Difference between revisions of "Part:BBa J10051:Experience"

(→‎Characterization of weak promoter (BBa_J23113) and strong RBS (BBa_B0031))
(→‎Applications of BBa_J10051)
Line 5: Line 5:
  
 
===Applications of BBa_J10051===
 
===Applications of BBa_J10051===
 +
Lambert 2019 used this part in experiments to model the effect of different promoter strengths and ribosomal binding strengths to develop a model to aid in controlling expression in constructs.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 15:28, 21 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J10051

Lambert 2019 used this part in experiments to model the effect of different promoter strengths and ribosomal binding strengths to develop a model to aid in controlling expression in constructs.

User Reviews

Lambert_GA 2019 Characterization

Characterization of weak promoter (BBa_J23113) and strong RBS (BBa_B0034)

T--Lambert GA--tuning3b.png


Figure 1. The highlighted points in yellow represent BBa_J10051 (weak promoter/ strong RBS) compared to BBa_J10050(weak promoter/ weak RBS) and BBa_J10052(weak promoter/ medium RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.



T--Lambert GA--tuning3a.png


Figure 2. The highlighted points represent the BBa_J10051 composite part (weak promoter/ strong RBS). As the promoter strength increases, the expression increases as well.


The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.




UNIQ528dae6c3d84baf8-partinfo-00000000-QINU UNIQ528dae6c3d84baf8-partinfo-00000001-QINU