Difference between revisions of "Part:BBa J10051:Experience"

(→‎Characterization of weak promoter (BBa_J23113) and strong RBS (BBa_B0031))
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===Applications of BBa_J10051===
 
===Applications of BBa_J10051===
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Lambert 2019 used this part in experiments to model the effect of different promoter strengths and ribosomal binding strengths to develop a model to aid in controlling expression in constructs.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 15:28, 21 October 2019


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Please enter how you used this part and how it worked out.

Applications of BBa_J10051

Lambert 2019 used this part in experiments to model the effect of different promoter strengths and ribosomal binding strengths to develop a model to aid in controlling expression in constructs.

User Reviews

Lambert_GA 2019 Characterization

Characterization of weak promoter (BBa_J23113) and strong RBS (BBa_B0034)

T--Lambert GA--tuning3b.png


Figure 1. The highlighted points in yellow represent BBa_J10051 (weak promoter/ strong RBS) compared to BBa_J10050(weak promoter/ weak RBS) and BBa_J10052(weak promoter/ medium RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.



T--Lambert GA--tuning3a.png


Figure 2. The highlighted points represent the BBa_J10051 composite part (weak promoter/ strong RBS). As the promoter strength increases, the expression increases as well.


The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.




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