Difference between revisions of "Part:BBa J10051:Experience"
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===User Reviews===
 
===User Reviews===
 
= Lambert_GA 2019 Characterization =
 
= Lambert_GA 2019 Characterization =
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==Characterization of weak promoter (BBa_J23113) and weak RBS (BBa_B0031)==
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==Characterization of weak promoter (BBa_J23113) and strong RBS (BBa_B0031)==
  
 
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Revision as of 15:23, 21 October 2019


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Please enter how you used this part and how it worked out.

Applications of BBa_J10051

User Reviews

Lambert_GA 2019 Characterization

Characterization of weak promoter (BBa_J23113) and strong RBS (BBa_B0031)

T--Lambert GA--tuning3b.png


Figure 1. The highlighted points in yellow represent BBa_J10051 (weak promoter/ strong RBS) compared to BBa_J10050(weak promoter/ weak RBS) and BBa_J10052(weak promoter/ medium RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.



T--Lambert GA--tuning3a.png


Figure 2. The highlighted points represent the BBa_J10051 composite part (weak promoter/ strong RBS). As the promoter strength increases, the expression increases as well.


The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.




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