Difference between revisions of "Part:BBa K3110010"
 
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This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a strong RBS.
 
This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a strong RBS.
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lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and  lower concentrations of lldD by varying  the strength of the promoter and RBS controlling its production.
 
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K3110010 parameters</partinfo>
 
<partinfo>BBa_K3110010 parameters</partinfo>
 
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<h1>Usage and Biology</h1>
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lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and  lower concentrations of lldD by varying  the strength of the promoter and RBS controlling its production.
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<h1> Characterization</h1>
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Due to time constraint and consecutive failures, we couldn’t make this construct and characterize it.

Latest revision as of 15:09, 21 October 2019


Weak Promoter Strong RBS lldD

This construct has L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and a strong RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1202
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 639


Usage and Biology

lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production.

Characterization

Due to time constraint and consecutive failures, we couldn’t make this construct and characterize it.